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How to Avoid Tears During Cryostat Sectioning

Slicing sections of tissues is a routine application in histology. Cryostat sectioning is one of the best methods to accomplish this. If not done properly, it can simply cause the tissue to melt, fold, curl, crack, or tear. We will address how to avoid tears. Tearing can occur if your blade is too dull or dirty, your damaged lead anti-roll plate needs replacement, or the temperature of your working environment is too low. Main reasons sections tear as they are being cut? The blade/knife edge is blunt or there is dirt, dust, frost or rust on the edge. The leading
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